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marylin monroe
Showing posts with label periworkout. Show all posts
Showing posts with label periworkout. Show all posts

22g High EAA (6g) Protein + 36g CHO Pre- / Intra-Workout Boost Fat Oxidation & PWO Resting(!) Energy Expenditure

I don't doubt that you can do that, too!
It does sound awkward: If you mix Twinlab: Amino Fuel (22 g protein - 6 g essential amino acids | L-phenylalanine: 633 mg; Lvaline: 781 mg; L-tryptophan: 133 mg; L-threonine: 679 mg; L-isoleucine: 565 mg; L-methionine: 292 mg, L-histidine: 282 mg; L-leucine: 1350 mg; L-lysine: 1449 mg) with a regular  sports recovery drink that contains 36g of simple sugar, down half of the resulting 800ml serving of whatever you want to call this mix immediately before your workout and consume the rest during the rest periods between sets, this will have measurable effects on your resting energy expenditure and fat oxidation.

From long-term to short time effects

At first, it does questionably sound counter-intuitive that the ingestion of an EAA + carbohydrate mixture before / during would increase the resting energy expenditure and rate of fatty acid oxidation after your workout. On the other hand, if you think about the long-term effects of corresponding supplement regimen, you don't have to look far, to find evidence that they can promote both, muscle gain and fat loss (Bird. 2006).
You can learn more about protein intake at the SuppVersity

Are You Protein Wheysting?

Cod protein for recovery

Protein requ. of athletes

High EAA protein for fat loss

Fast vs. slow protein

Too much ado about protein?
Kyle J. Hackney, Andrew R. Kelleher, and Lori L. Ploutz-Snyder from the Syracuse University speculated that the highly beneficial changes in body composition Bird et al. observed in their study participants over the course of a 12-week strength training + EAA & CHO supplementation that after "[t]hese adaptations may be related to the acute energy expenditure and substrate utilization responses in the postexercise period." (Hackney. 2013)
Figure 1: The changes in body composition (in kg) in response to 12 weeks of resistance training + placebo, CHO, EAA or CHO + EAA supplementation in 2006 study by Bird et al. "inspired" Hackney et al.
Against that background, it was only logical to conduct a study to examine how multiple bouts of resistance exercise with and without the strategically timed intake of amino acids affect the resting energy expenditure (REE) and respiratory exchange ratio (RER). The results could after all explain if the long/er) term effects on body composition that have been observed in previous studies using chronic training and supplementation regimen are maybe nothing but necessary consequences of repeated acute increases in REE or decreases in RER (you hopefully remember that a decrease in the respiratory exchange ratio signifies an increase in fatty acid oxidation).

Experimental design and results

To this ends, the researchers recruited 10 young (mean age: 23.4y) recreationally trained male participants. All of them had been participating in general resistance training exercise for a minimum of 3 days per week for at least 6 months.
Figure 2: Changes in resting energy expenditure (kcal/day) and comparison of training volume in 58g CHO (black bars) and EAA + CHO (white bars) trials (Hackney. 2013).
As you can see in Figure 2, Hackney et al.'s original hypothesis that "intake of amino acids with each resistance exercise session would lead to greater perturbations of REE and RER" (Hackney. 2013) does unquestionably hold for this population of average (rookie) gymrats.

Whether the scientists "main finding" (Hackney. 2013), i.e. the 3.61% increase in resting energy expenditure (REE) will be similarly pronounced in advanced trainees is yet as questionable as the real-world effects of this artificial value. Despite the fact that Hackney et al. are right, when they say that our resting energy expenditure "represents the largest component of [our] total daily energy expenditure (60–85%) and has been implicated as a major contributor to overall body mass management " (Hackney. 2013), I am not sure how "major" an increase of only 66kcal per day actually is... I mean,  if this pathetic increase in resting energy expenditure was the actual driving force we would need almost 100 days to shed a hilarious pound of body fat (note: the reason I use the flawed 3,500kcal = 1lbs of fat rule of thumb here is that the whole REE calculations would be pointless if you didn't put at least some faith into the "energy in vs. energy out" hypothesis of weight loss - right?)
SuppVersity Suggested Read: "Fat Loss Principles That Work: 10g+ of EAAs W/ Every Meal. Do Energetic Costs of Protein Synthesis Trigger This Effect?" |  read more
Bottom line: It stands out of question that your training success can benefit from a high EAA protein source and some carbs you consume before and during the exercise session. Whether the fat loss benefits are actually brought about by the marginally increased resting energy expenditure (REE) is yet something I doubt - it certainly helps fat loss, but clearly isn't its main motor.

Don't get me wrong, this does not imply that you will benefit from this type of "peri-workout" supplementation. And let's be honest, the end most of you probably don't care about the exact underlying mechanisms, as long as your body composition keeps improving, right?
References:
  • Bird, S. P., Tarpenning, K. M., & Marino, F. E. (2006). Independent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men. European journal of applied physiology, 97(2), 225-238.
  • Hackney, K. J., Kelleher, A. R., & Ploutz-Snyder, L. L. (2013). Amino Acid-Carbohydrate Intake Combined with Multiple Bouts of Resistance Exercise Increases Resting Energy Expenditure. ISRN Nutrition, 2013.

Creatine Before or After the Workout? Finally the Answer is Here! Study Says: Better Take it After for Mass & Strength

The old "creatine = water retention" myth is - at least for the majority of athletes - just that: a myth and a result of the old practice of "loading" with copious amounts of creatine and even more high GI carbs. And it is certainly not a problem that's specific to cheap and effective creatine monohydrate.
In case someone of you ever asked me, whether he or she should take his or her creatine before or after a workout I will probably have answered something like: "I don't think it will make much of a difference, but I personally would suggest you split the dosage".

Now that Jose Antonio and Victoria Ciccone (the first of whom many will probably know from his old radio show on BB.com, the countless articles he wrote, his job as an editor or whatever else), published a paper in the Journal of the International Society of Sports Nutrition, it appears my new answer to the said question would be: "It looks like it's better to take your creatine after a workout." My previous suggestion to take 50% before and 50% after, on the other hand, has not yet been falsified. So maybe I will have to go back on the revised advice again and re-revise it, so to say. And you know what? That's not a problem! That's science. Any "truth" in science is only true until it has been falsified and I do already have my doubts about the universality of this new "truth" about creatine (see bottom line).

So, if after is the way to go? How do we know?

What Antonio and Ciccone did was to recruit 19 male recreational bodybuilders (mean ± SD: age, 23.1 ±2.9 years; height, 166.0 ± 23.2  cm;  body  weight,  80.2  ±  10.4  kg) with training experience of  >1 year who were obliged to stop taking any form of workout supplement or dietary aid at least 4 weeks before the study began.
The Pharmacokinetics of creatine Part I & Part II
"Subjects were randomly assigned to one of two groups: a PRE-SUPP or POST-SUPP group. The PRE-SUPP group consumed 5 grams of creatine monohydrate immediately prior to training. The POST-SUPP group consumed the same amount of creatine immediately after training. Following pre-testing data collection, participants began a periodized four-week resistance training program that was self-administered. On off-training days, subjects consumed creatine at their convenience. The total treatment duration was four weeks." (Antonio. 2013)
All subjects participated in the same standardized periodized, split-routine bodybuilding training regimen that was "geared primarily for skeletal muscle hypertrophy" (Antonio. 2013).
  • training frequency: 5x per week
  • total number training sessions: 20 
  • training duration: ~ 60 min per session
As you can see in the overview in figure 1 the training protocol used a block periodization with a change in the rep scheme after each cycle (=4 workouts, one body part is worked in the respective rep range once) and a pretty high training density, i.e. 5 workouts in a row + 2 days off.
Figure 1: Outline of the training schedule; created based on the description on the original study (Antonio. 2013)
Certainly not exactly a beginner program, but with ~60min per workout it's feasible to train like this for someone who is not getting all too stressed up in his everyday life and has the training experience to handle the CNS load.

That's the study you've been waiting for, right?

With experienced trainees, the requirements of not ingesting any other supplements (including amino acids) that would probably thwart the results, standardized strength tests, as well as random 24h dietary recalls every week, obligatory training logs and BodPod body fat measurements, this is the kind of study of which I would love to see at least once a week - not every 6 months.
Figure 2: Changes in body composition and 1-RM bench press strength, left; nutrient composition in grams/day, right (Antonio. 2013)
The results, however, point towards the main reason studies like these are rare. The chance to measure significant effects in trained individuals are much lower than they are in the average couch potato who grows and drops fat like a maniac, at the very moment he is shooed around in the gym.

In the bodybuilders in the study at hand only the changes in fat free mass and the bench press power did reach statistical significance and the superiority of the post-workout supplementation is "possible" and "likely", but by no means certain. Still, Antonio and Ciccone feel that
Additional tip: Supercharge creatine with baking soda (learn more)
"[t]he use of recreational bodybuilders in the current investigation is advantageous because it is difficult for highly trained individuals to experience an increase in FFM or muscular strength in the time frame allotted for this study." (Antonio. 2013)
Moreover, they point out that "of the 19 subjects that completed the study, 16-21% were non-responders regarding muscular strength and FFM." That's irrespective of the nutrient intake, by the was which was similar between the groups and had with 1.9g of protein per kg body weight per day more than enough protein to support muscle growth.

So is it effective or not?

For an optimal ratio of lean to fat mass loss on a diet it takes "only" twice the RDA of protein.
With respect to the effectiveness of the creatine supplement, the high protein intake could, as Antonio and Ciccone speculate even have been a disadvantage as this could mean that the subjects "could already have a high amount of creatine stored intramuscularly and this may have blunted the results" (Antonio. 2013).

How's that? Well, not only as a result from the minimal amount of creatine in beef, but also due to our bodies ability to produce creatine from L-arginine, glycine, and L-methionine - all of which the trainees should have gotten plenty in their diet.

If we also assume that some of the guys have been "on" creatine before, 4 weeks is not enough to deplete the stores if you don't resort to guanidinoproprionic acid (GPA) to deliberately deplete them (learn more about GPA).

Bottom line: The benefits may have been subtle, but in view of the fact that we do not have evidence to the contrary you better make sure you take your creatine after your workouts from now on, or all the work you're putting into your workouts is going to be lost... just kiddin', I personally still believe that it does not matter. Creatine uptake is slightly higher with some insulin floating around and the degradation is lower when it does not reside too long in the acidic millieu of the stomach etc. So, without any more specific information on whether or not the subjects consumed their creatine with a meal before or after the workout and whether there were carbs, fiber and whatever in that meal, it's really premature to say whether or not (a) the timing really makes a difference and (b) whether post-workout supplementation is the only way to go.

References:
  • Antonio J, Ciccone V. The effects of pre versus post workout supplementation of creatine monohydrate on body composition and strength. J Int Soc Sports Nutr. 2013 Aug 6;10(1):36. [Epub ahead of print]

Peri-Workout Hydro-Whey Supplementation: Tried & Proven Muscle Builder Will Also Increase Tendon Size & Strength

Squats are by no means the only exercise that requires strong muscles and tendons.
In a way you could argue that today's SuppVersity article serves two purposes. Firstly, it provides direct evidence for my outrageous claim that the VPX Shotgun + Synthesize study from Saturday's installment of On Short Notice has little to no practical value, because the use of 2x17g of maltodextrin as a control has little to do with the real-world supplementation regimen the potential consumers of respective products are ingesting. I mean, you can safely assume that people who are willing to spend the money on expensive peri-workout kitchen sink supplements will - just like most of you - be ingesting a whey (or other fast acting) protein source after their workouts, anyways.

It goes without saying that it would not have needed a new study to prove this point, so that informing you about another observation Farup et al. made in their most recent study is not just the second, but actually the main purpose of today's SuppVersity article.

A new whey to build tendons of steel

The training protocol: The subjects completed 33 training sessions during the 12 weeks of training. The training program was primarily designed to induce hypertrophy and to a lesser extent maximal muscle strength highlighted by a large number of sets and repetitions and by moderate high intensity. Training frequency was three times per week with a progressive increase in volume and intensity throughout the 12 weeks.
The resistance training exercise consisted of isolated knee extensions in a Technogym knee extensor machine. All repetitions were performed by lifting the load with the concentric leg (while extending the
eccentric leg unloaded). Then, with aid from a training supervisor, an additional load was released onto the weight stack and then lowered with the eccentric leg.
Both the eccentric and concentric leg training program consisted of isotonic knee extensions (TUT 2-0-2s; rest between sets 2min) and applied the following progression in volume and intensity: 6 x 10-15RM (sessions 1–4), 8 x 10-15RM (S 5–10), 10 x 10-15RM (S 11–20), 12¥6–10 RM (S 21–28), and 8 x 6-10RM (S 29–33).
The actual paper is going to be published in one of the future issues of The Scandinavian Journal of Medicine & Science in Sports and deals with the outcomes of a 12-week double-blinded resistance training intervention in the course of which the 22 male subjects (healthy, young, recreationally active height 181.5+/- 1.5 cm, weight 78.1 1.8 kg, age 23.9 +/-0.8 years, fat% 16.0+/- 0.9%) consumed either
  • a high leucine whey protein hydrolysate + carbohydrate group (WHD; 19.5 g whey protein hydrolysate +19.5 g) or 
  • an isoenergetic carbohydrate only supplement for the placebo group (PLA, 36g of carbohydrates)
Regardless of supplementary intake, all subjects performed eccentric training with one leg and concentric training with the other.
"This within-subject design was used to minimize the potential differences in the hypertrophy response that are inherent with group designs (e.g. initial training status, habitual nutritional intake,and/or hormonal status). Eccentric leg was randomly chosen to be either the dominant (preferred kicking leg) or the nondominant leg to exclude any potential pre-training difference between the two." (Farup. 2013)
Overall, we are thus comparing not two, but rather four different training modalities, namely eccentric+WHD, eccentric+PLA, concentric+WHD and concentric+PLA.

Maximal standardization, reliable measuring methods

In the two weeks before commencing the training program, magnetic resonance image (MRI) scans of both thighs and patellar tendons and isometric strength test were taken. In conjunction with the before and after tests that were standardized up to the time of the day, pre- and post-training, "to control for potential effects of diurnal rhythm" (Farup. 2013)

The training sessions were closely supervised and monitored by qualified training instructors to ensure proper execution and loading. The same goes for the ingestion of the supplements, 19.5 g whey protein hydrolysate +19.5 g of carbohydrate (both equal to 4% solution) and the placebo drink consisting of 39 g of carbohydrate, which were handed to the trainees at the beginning of the training sessions and were to be consumed before and after the training (50/50).

Even the intake of additional plain water was standardized, so that the subjects would not ingest and fluids 1 1/2 h prior to and 1 h after completion of an exercise session, "to ensure and standardize the conditions for digestion/absorption and within the range typically applied" (Farup. 2013)

The only methodologically lapse was the absence of dietary control. While the subjects of previous studies in which the participants were advised to "maintain their normal habitual dietary intake throughout the study" (Farup. 2013), did not register any differences in habitual total energy or protein intake (Andersen. 2005; Hartman. 2007; Hulmi. 2009; Erskine. 2012) and the accuracy of food logs is generally questionable, this is kind of awkward in view of the lengths to which the scientists went to exclude any other confounding factors.

Enough of the prelude, what about the results?

So, while we cannot exclude that the subjects in any of the two groups may have skewed the overall results by consuming an additional steak on top of their regular diets, it is quite unlikely that these counfounding factors would have been group specific, so that the overall effects on quadriceps cross-sectional area I plotted in figure 1 are unquestionably reliable.
Figure 1: Relative changes in quadriceps and patella tendon CSA in concentrically and eccentrically trained leg of the subjects in the whey protein hydrolysate and placebo groups (Farup. 2013)
The same obviously goes for the strength increases and the initially mentioned increase in patella tendon CSA. All good and reliable evidence supporting the current "standard" in periworkout supplementation.

Apropos, in conjunction with the recent revelations about the unique glucose-sensitizing effects of the short-chain peptides in whey hydrolysate, it may even be worth to consider adding a "hyrolysate" to the current recommendation to ingest 30g of whey protein in the vicinity of your workouts. Convincing evidence from studies that were specifically designed to elicit the marginal benefits of replacing a regular whey protein with the less tasty (you won't notice that with the tons of flavoring agents, though) and still more expensive "pre-digested" form of whey is yet - at least as far as I know - still missing.



Bottom line: Actually, I already mentioned the most important findings of the study at hand in the introduction:

Figure 2: Whether the effects observed in the study are "hydrolysate specific" and related to the amino acid or peptide composition of the supplement that was used in the study at hand cannot be said without a "regular" whey control. The same goes for the general superiority or inferiority of hydrolysates vs. whey isolates or concentrates.
(a) Whey proteins alone will amplify the effects of regular strength training to a degree that is hard to surpass by the more expensive "advanced muscle builders" - no wonder the producers are reluctant to use anything but an isocaloric carbohydrate supplement as their yardstick. (b) Increases in tendon CSA are a novel benefit to be added to the comprehensive list of benefits of a supplement I suspect most of you are using anyway.

Whether the hydrolyzed whey proteins are actually worth the extra bucks is yet still not 100% certain. As previously mentioned, the number of practically relevant direct comparisons is still scarce. We know that they create a more rapid increase of the amino acid levels in the blood, that they are more insulinogenic and we that some of the short peptides have favorable physiological effects (learn more). A long-term study comparing the muscle and (that's new) tendon building effects of the three commonly available varieties of whey, i.e. concentrate, isolate and hydrolysate, has not yet been conducted. But don't worry, as soon as the pertinent data is going to be available, you'll find all the information you need, as well as the practical implications of the results right here, at the SuppVersity - your #1 source for the latest on exercise, nutrition and supplementation research on the Internet.

References:
  • Farup J, Rahbek SK, Vendelbo MH, Matzon A, Hindhede J, Bejder A, Ringgard S, Vissing K. Whey protein hydrolysate augments tendon and muscle hypertrophy independent of resistance exercise contraction mode. Scand J Med Sci Sports. 2013 May 7.

Cod Liver Oil vs. URTI. 2x Higher Fat Oxidation W/ Optimal Workout Timing, Aggressive Diabesity Control, Seaweed & Breast Cancer and NO Supplements Superior to Sugar

Self-proclaimed experts will say: "No cod liver! It's poison! It has way too much vitamin A... you better pop a kg of vitamin D pills if you want to do something for your health." Really? How do you explain the results of the Robertson study, then?
    "Grandma was right" - That's if you will the statement of the week - a statement that is verified by the SuppVersity Figure of the Week which is p = 0.04 and describes the probability that the association between the consumption of cod liver oil and a decreased occurrence of upper respiratory tract infects (URTI) Swedish researchers observed in a cohort of 6350 middle-aged and elderly participants in the Tromsø Study 6, of which Robertsen et al. had actually expected that it would finally confirm the protective effects of high(er) vitamin D levels / intake against respiratory disease. As it turned out, however, neither vitamin D (25-OHD), nor the intake of fish, n-3 capsules or vitamin and/or mineral supplements were significantly associated. Shamed be he who thinks of vitamin A now ;-)
    • 2-Fold Increase in Fat Oxidation W/ Correct Workout Timing (Darvakh. 2013) -- If we assume that you want to burn the maximal amount of fat during a workout, timing could be a more important factor than previously thought.
      Figure 1: Maximal fat oxidation, intensity necessary to achieve the maximal fatty acid oxidation and total fat oxidation per minute; all values expressed relative to absolute mean in each category (Darvakh. 2013)
      In a recent study from the Department of Physical Education and Sport Sciences at the Shahid Chamran University of Ahvaz in Iran the maximal rate of fatty acid oxidation in healthy, but untrained subjects was 2x higher in the afternoon compared to both the morning and evening conditions.

      What's likewise interesting to observe is the fact that the exercise intensity that was required to achieve maximal fatty acid oxidation was higher in the morning. No wonder that the total fatty acid oxidation per minute in all three trials was the highest, when the participants worked out in the morning!
      Bottom line: It's not just the missing dietary control, but also the mere idea that "burning fat for fuel" during a workout would have beneficial effects on long-term weight loss - let alone help you shed weight, when you are not in a caloric deficit that renders the results of the study at hand interesting, but more or less irrelevant. My personal advice would thus be: Work out, whenever you can muster the time and whenever you got the choice, base your decision on when to work out on performance measures, not results from studies like these (learn more about planning your workouts here).
    • Is the time ripe for aggressive diabesity cures?! (Belsare. 2010) -- Everyone who has listened to the Wednesday episode of Super Human Radio, when Carl Lanore interviewed the Indian researcher Milind G. Watve, co-author of the paper at hand, will already be familar with the concept:
      Table 1: Molecules that affect aggressive behavior as well as insulin resistance (Belsare. 2010)
      Physical aggression is known to increase secretion of epidermal growth factor (EGF) in anticipation of injuries and EGF is important in pancreatic beta cell regeneration too. In anticipation of injuries aggression related hormones also facilitate angiogenesis and angiogenesis dysfunction is the root cause of a number of co-morbidities of insulin resistance syndrome.

      Reduced injury proneness typical of ‘diplomat’ life style would also reorient the immune system resulting into delayed wound healing on the one hand and increased systemic inflammation on the other. Diabetes is negatively associated with physically aggressive behaviour."
      These and the unquestionably striking associations the researchers collected in the tabular overview I used as a basis for table 1 to the right of this post (you read it a follows: arrows indicate up-/down-regulation or pos-/neg. correlation; "A"/"B" causation / reverse causation shown in A1/B1 = mutant or knockout experiment; A2/B2 = pharmacological/physiological exp. based on infusion or use of agonists/antagonists); C indicates only statistical association w/out direct evidence for causality), would unquestionably justify the hypothesis:
      "[...]that suppression of physical aggression is the major behavioural cue for the development of metabolic syndrome."
      However, despite the fact that "preliminary trials of behavioural intervention indicate that games and exercises involving physical aggression reduce systemic inflammation and improve glycemic control" - the same is true for "unagressive" types of exercise, so that I can't but vouch my doubts about the causative nature of aggression in this game of convenient laziness.
      Sugg. Podcast: Doves, Diplomats & Diabetes: A Darwinian Interpretation Of the Obesity Epidemic (listen)
      Bottom line: Irrespective of the causative or correlative nature of the lack of aggression or its presence in the etiology and resolution of the obesity epidemic.

      In the end, I am with Dr. Watve and his Indian colleagues: the non-pharmacological and in my humble opinion only viable solution to our problem are lifestyle changes - and as far as the "aggressiveness" is concerned, I guess, we'd just have to enforce these more aggressively. At least as aggressively as the Australians are leading their "war against" cigarette smoking - maybe a "war against" and not as a quest for revenue-generating pharmacological solutions to behavioral problems as we are still doing it by now.
    • The breast cancer protective effects of seaweed - proof of principle (Teas. 2013) -- Researchers from the South Carolina Cancer Center at the University of South Carolina state in their recently published paper in the Journal of Applied Physiology that the consumption of seaweed (Undaria) in the form of a capped supplement (5g/day, total) decreases the urinary human urokinase-type plasminogen activator receptor (uPAR) concentrations in15 healthy postmenopausal women (5 had no history of breast cancer, 5 were breast cancer surivors) during a 3-month single-blinded placebo controlled clinical trial.
      What to make of these results? With the well-established increase in uPAR in post-menopausal and it's highly significant correlation with unfavorable breast cancer outcomes. The scientists argue that this controlled experiment would provide the hitherto missing "proof of principle" to confirm the epidemiologically observed anti-breast cancer effects of seaweed.
    • Pre- & Post-workout supplementation with VPX' flagship products NO Shotgun & Synthesize promote fat free gains more than pure sugar (Ormsbee. 2013) -- Actually I did not even want to post this study, but it's so hilariously funny that I could not resist informing you about the marginal edge the provision of the VPX pre- & post-workout supplements NO Shotgun & NO Synthesize had in a sponsored study from the Florida State University that's been published in provisional form on Friday afternoon.

      When the researchers analyzed the effects the provision of the said supplements before and after each of the workouts the 29 healthy resistance trained men had compared to a maltodextrin [yeah, that's protein, BCAA, creatine, caffeine, beta alanine & more in the "multi-ingredient performance supplements (MIPS) vs. plain sugar as "control" placebo)] they found
      • Figure 2: Body composition of previously resistance trained subjects after 6 weeks of standardized thrice weekly progressive strength training in participants ingesting one serving of NO Shotgun and Synthesize (MIPS) vs. 2x17g maltodextrin (placebo) pre / post workout; values expressed relative to group baseline (Ormsbee. 2013)
        no group time  interaction  for  HR,  BP,  blood  glucose,  lipids,  NOx,  hs-CRP,
        cortisol  concentrations  or  body  fat
      • significant decrease in body fat in both groups (mean ± SD; MIPS: -1.2 ± 1.2%; Placebo: -0.9  ± 1.1%), android fat (MIPS: -1.8 ± 2.1%; Placebo: -1.6 ± 2.0%), and gynoid fat (MIPS: -1.3 ± 1 .6%; Placebo: -1.0 ± 1.4%) and *drum rolls please* 
      • a  group × time interaction  for the increases in fat-free mass which were significantly  higher (4.2% vs. 1.9%) after the ingestion of the kitchen sink supplements compared to the 21g of pure maltodextrin
      What? Yeah, I know that VPX has had a very similar study done in the past (read up on the details, here) and obviously nobody read my comment back in the days that it's nice to spend some money to prove that your supplements works, but basically useless if the study protocol is designed in a way that ensures that your product will come as the more or, as in this case, rather less glorious winner.

      Bottom line: I wonder when the first supplement company dares doing a study comparing their product to a basic comination of creatine + whey and a cup of coffee before the workouts... what? Oh, you suspect that this could hamper their sales? Well, I guess you could be right.



      That's it for this installment of "On Short Notice"!  If you still have to kill some time before you start into the weekend, I suggest you take a peek at one of the following Facebook news:
      • I hope you did not already forget that. The isoleucine-containing peptides in hydro-whey can also ramp up GLUT-4 expression and increase skeletal muscle glucose uptake - a true repartitioning effect (learn more)
        Endurance boosting effects of hydro whey -- Whey protein hydrolysate aka "low molecular weight whey" has superior effects on the endurance of rodents compared to regular whey isolate (read more)
      • Influenza: marketing vaccine by marketing disease -- Young Harvard scientists speaks out about the hilariousness of the flu vaccine (learn more)
      • Further evidence androgens program fat-cells-to-be to become bone, not fat -- "[O]ur results suggest androgens promote an osteogenic gene program at the expense of adipocyte differentiation." (read more)
      • Metabolic IN-flexibility is a characteristic feature of PCOS in women -- That's corroborated by both insulin resistance and androgen excess (learn more)
        If that's still not enough to satisfy your cravings, just "like" the SuppVersity on Facebook and keep on par with the latest news of which I am sure there will be more to come before the next full article is going to be published tomorrow.

            References:
            • Belsare PV, Watve MG, Ghaskadbi SS, Bhat DS, Yajnik CS, Jog M. Metabolic syndrome: aggression control mechanisms gone out of control. Med Hypotheses. 2010 Mar;74(3):578-89.
            • Darvakh H, Nikbakht M, Shakerian S, Mousavian AS.  Effect of Circadian Rhythm on Peak of Maximal Fat Oxidation on Non-Athletic. Zahedan J Res Med Sci. 2013; 15 [epub ahead of print].
            • Ormsbee MJ, Thomas DD, Mandler WK, Ward EG, Kinsey AW, Panton LB, Scheett TP, Hooshmand S, Simonavice E, Kim JS. The effects of pre- and post-exercise consumption of multi-ingredient performance supplements on cardiovascular health and body fat in trained men after six weeks of resistance training: a stratified, randomized, double-blind study. Nutr Metab (Lond). 2013 May 16;10(1):39.
            • Robertsen S, Grimnes G, Melbye H. Association between serum 25-hydroxyvitamin D concentration and symptoms of respiratory tract infection in a Norwegian population: the Tromsø Study. Public Health Nutr. 2013 May 9:1-7.  
            • Teas J, Vena S, Cone DL, Irhimeh M. The consumption of seaweed as a protective factor in the etiology of breast cancer: proof of principle. J Appl Phycol. 2013 Jun;25(3):771-779.

              Short-Term Supplementation with Bovine Colostrum Does Not Improve Immune Variables in Well-Trained Athletes. Localized Effect on Intestinal Health Yet Possible.

              Just in case you wonder that there were no news yesterday. Blogger was down, so it took me some time to be able to tell all of you, who followed my advice and tuned in to yesterday's episode of Carl Lenore's Super Human Radio: "Amino Acids for Super Humans" and heard Carl and me talk about his use of bovine colostrums as part of his peri-workout supplementation regimen that my concerns over the digestibility of the (mostly) immunomodulating long-chain peptides in colostrum (the milk mammals produce in the last days / first days after pregnancy), appear to be legit. At least the results of a recents study (Carol. 2011), which failed to produce any (systemic) immunomodulatory effects in well-trained athletes, would confirm my assumption that these longer amino acid chains are not able to overcome the tight gut junctions, which are genetically designed to "close" (the further tightening of the junctions is also known as "closure") within the first days after a mammal is born.
              Figure 1: Two samples of human breast milk, which has a very different immunoglobulin composition than cows milk and colostrum, cf. Hurley. 2011 (image taken by Wikipedia user Azoreg)

              That being said, the results of the aforementioned study that has been published in International Journal of Sports Nutrition and Exercise Metabolism do not come as a surprise for me: After 10 days of supplementation with either colostrums or skim milk powder, the 9 professional male athletes did show absolutely identical immune reactions to high intensity endurance exercise in a carbohydrate depleted state (the glycogen-depletion would augment the immunosuppressive effect of intense exercise beyond what would be seen under normal = glycogen-sufficient circumstances):
              Plasma cortisol levels increased over time, reaching the highest level directly after exercise, and were still elevated ~22 hr after exercise compared with baseline values (p < .001). Neutrophil cell count was increased after exercise and dropped below starting values 22 hr after exercise (time effect p < .001). Circulating immunoglobulins did not change over time. A significant time effect was seen for interleukin (IL)-6, IL-10, IL-1-receptor agonist, and C-reactive protein, with levels being higher directly after exercise (p < .05). Other cytokines (interferon-γ, IL-1a, IL-8, tumor necrosis factor-a) did not show a time effect. No differences were seen between colostrum and skim-milk powder in any of the investigated variables.
              The lack of systemic effects, i.e. effects on the body as a whole, due to the inability of the long chain peptides to cross the intestinal wall, on the other hand, does not preclude beneficial "side effects" within the gut, itself. Recent studies such as Moller et al. (Moller. 2011), for example showed direct beneficial effects of the "synergistic action of various milk bioactives" on dendritic cell (messengers between the innate and adaptive immunity) cytokine response within the gut. Further evidence for the localized effect of colostrum comes from a 2010 study by Marchbank (Marchbank. 2010) found that colostrum supplementation actually decreased or rather maintained gut permeability, which was otherwise reduced after exhaustive exercise:
              Intestinal permeability in the placebo arm increased 2.5-fold following exercise (0.38 ± 0.012 baseline, to 0.92 ± 0.014, P < 0.01), whereas colostrum truncated rise by 80% (0.38 ± 0.012 baseline to 0.49 ± 0.017) following exercise.
              These colostrum-specific results corroborate various studies on the effects of hydrolyzed milk peptides on insulin sensitivity, blood pressure and much more, indicate that there is much more to milk and milk products than the sum of their proteins, carbohydrates, fats, minerals and vitamins would suggest. Whether it always has to be colostrum, which is expensive and hard to come by, or raw, unpasteurized milk from the grass-fed cows of your local farmer would not suffice remains to be seen, though.

              Sip Casein(-ate) NOT Whey for Maximal Net Protein Retention? Not Despite, but Due to Less Leucine?

              Image 1: Cows must be stupid animals, right? I mean everybody knows that whey is better than casein, so why would they "spike" their milk with this allegedly inferior protein source, instead of providing us and their calves with the "most anabolic", leucine-loaden whey protein? Or do cows know better than us?
              Question: "Whey or Casein to maximize protein synthesis?" The answer appears to be easy: Whey, of course! Faster absorption, higher rates of hyperaminoacidemia (higher than normal amino acid levels in the blood), more leucine, more mTOR, more insulin, more, more ... and did I say more? Well, other than maybe Dr. Scott Connelly, who just came out with his new total milk protein based meal supplement product Physique 2.0, you will in fact be hard pressed to find anyone, let alone a renowned expert arguing in favor of casein. Yet still, Marielle P.K.J. Engelen and her colleagues from the University of Arkansas for Medical Science, Maastricht University, the Nutrition and Toxicology Research Institute Maastricht (NUTRIM),and the Federal University of Rio de Janeiro (UFRJ) dared conducting a trial which investigated just that (Engelen. 2012): "Which dairy protein is able to positively influence the protein metabolic response to exercise" - unfortunately in COPD patients.

              COPD vs. control - different yet the same

              Luckily the combined exercise and supplementation protocol in the course of which the 8 COPD patients and their age-matched (68.3y) healthy controls had reported to the lab fasted, where they - after the usual procedure of setting up a catheder, infusing a tracer, etc. - started consuming one of the following enteral protein meals
              • 29.5 g sodium caseinate* and 68.5g of maltodextrin, or
              • 29.5 g whey protein and 68.5g of maltodextrin
                *please make sure you read the info on caseinate vs. casein in the red box below
              both dissolved in ultrapure water (1L) at 20 min intervals beginning at T = -2h; in plain English "two hours before the actual trial begun. In the course of the latter the healthy subjects (on which we are going to focus our attention here) pedaled on braked cycle ergometer at 50% of the COPD patients pre-determined maximal workload (this was a concession to the bad conditioning of the actual study subjects, the COPD patients) in the first and 50% of their own maximal workload in the second trial at T = 0 and T = 1h 20min, respectively.
              Image 2: Don't be fooled micellar casein and caseinate are not the same (img. University of Guelph)
              Caseinate is not casein! I have not discussed the actual study in isolation, here at the SuppVersity before, but I have broached on the issue in previous posts: There is more than a "three-letter difference" between "casein" and "caseinate", while the former actually (often things get "mislabeled" these days ;-) refers to intact casein micelles, which clot and form complex novel (and partially biologically functional) complexes in the course of the digestion process, the sodium bound "broken" micelles from the sodium caseinate don't, so that - as Stuart Phillips put it quite aptly in a comment on a similar study by Reitelseder et al. - the "digestion rates of this form of casein are not likely to be overtly different from those of whey" (Phillips. 2011). What we are comparing here are thusly proteins with "identical", or I should say, very similar digestion rates, yet slightly different amino acid compositions - this renders the study not less valuable, should yet be kept in mind as far as its practical implications (see my conclusion further down) are concerned.
              Right before, as well as right after, and in the course of the 60min recovery period that followed each workout the subjects consumed a bolus of 0.67 mL/kg BW of the respective protein supplement which contained 18 mg protein/kg body weight (whey or caseinate) and 46 mg maltodextrin/ kg BW every 20 min. Afte 10 ingestions and a 75kg subject would thusly have consumed 13.5g of protein, total.
              Figure 1: Total body net protein synthesis (synthesis - breakdown) and protein synthesis of the healthy subjects during (0-20min and 80-100min) and after the two exercise trials (data adapted from Engelen. 2012)
              And even in view of the fact that we are not dealing with regular slow digesting casein micelles, the results, in terms of net protein retention and protein synthesis (cf. figure 1) were quite surprising:
              The present study shows that net whole body protein anabolism was higher during casein than during whey protein feeding in both the COPD and control group which remained during and following exercise. The proteins were provided via sip feeding to evaluate the effects of the quality of the amino acid composition between casein and whey protein. [...] This difference in anabolism might be related to differences in BCAA distribution as LEU level is higher in whey than in casein protein, whereas the concentrations of ILE and VAL are lower
              And though I am not quite sure, whether the researchers were aware that "their" caseinate is in fact not significantly slower digested than regular whey protein, this does not change the likeliness of their hypothesis (= hypothetical ;-) that the different amino acid make-up in general and maybe even the lower (!) leucine to isoleucine and valine ratio could be responsible for the statistically significant superiority of caseinate in
              • whole body net protein synthesis during exercise and recovery
              • whole body protein breakdown during recovery from the second, higher intensity exercise bout, and
              • net whole body protein synthesis (=difference between synthesis and breakdown) during the whole study period, with the exception of recovery from 2nd exercise trial
              Whether or  not this was a (direct) effect of the different ratios of the three BCAAs to each other, the minimally higher BCAA and EAA content (btw. why not the proline? I mean 11.23 in caseinate vs. 3.31 is a huge difference, cf. table 1) of the caseinate or maybe, despite similar digestion rates, still an effect that came about as a result of different digestion kinematics of whey and caseinate would require further studies.

              Is whey no longer the whey to go?

              Table 1: Amino acid composition (per 100g) of the sodium caseinate and whey used in the study (Engelen. 2012)
              In view of what you should have learned yesterday about the influence of age, training status, experimental protocol, etc. on the effect-size of studies investigating strength gains in response to different exercise regimen (cf. Strength Gains Depend on Training Status, Age, Workout Frequency, Rest Intervals & More), it should also be clear that these results require further verification in different study populations and exercise contexts. It is yet still intriguing that - assuming that the scientists hypothesis holds, two of the generally accepted paradigms of intra- and peri-workout supplemenation, namely
              1. the general superiority of whey proteins over every other form of dairy (let alone meat or whatever else) protein during or in the immediate vicinity of a workout
              2. the importance of leucine as the single-most important dietary trigger for protein synthesis and against the breakdown of protein
              If these paradigms were revised, the currently available 4:1:1 or even 8:1:1 BCAA supplements, as well as the whey-protein based intra-workout supplements, should (you know science is only part of the equation, when it comes to the production and sales ranks of dietary supplements ;-) disappear from the market.

              Comparing apples to oranges and juices to whole fruits

              Before the latter is going to happen, though, there is still a lot of scientific work to be done. After all, we are not only comparing apples with oranges here (as far as the trainees are concerned), but also juices with whole fruits, or put more simply: While the current study looked at whole body protein synthesis / breakdown, sports scientists usually focus on muscular (in many cases even either myofibrillar or sarcoplasmic) protein synthesis, the study at hand evaluated the protein turnover rates of the whole body. And while this is probably a "shortcoming" as far as the immediate muscle-building effects of the individual protein sources are concerned, the ever-increasing awareness of the far-reaching metabolic consequences of the integrity of our gut lining, which becomes a protein donor for the skeletal muscle tissue during strenuous exercise (after a workout you have a "leaky gut"), may make it worthwhile to look at protein synthesis in places other than your muscle as well. After your workout, at the latest, your gut and the other organs in the splachnic bed are going to (re-)claim their share of protein, anyway.

              Triphasic Nutrient Supplement W/ Caffeine, Aminos, Carbs, Creatine & All the Usual Suspects Allows For Higher Training Volume, Lowers Cortisol & Dampens Muscle Damage

              Do you really need the whole pre-, intra-, post workout supp-arsenal to benefit from your workouts?
              Finally, another workout supplementation study! Yeah, I know you are already suffering the side-effects of withdrawl, but it's just a couple of lines and you will feel relieve - thanks to Stephen P. Bird and his colleagues from the Charles Sturt University in Bathurst, Australia, whose latest paper is about to be published in one of the upcoming issues of Nutrition Research (Bird. 2013). And as if that was not already enough, the participants were strength trained athletes, the  physical activity and diet were standardized according to pre-recorded habits and the supplementation protocol was extensive! With pre-, intra- and post-workout supplements it was exactly what many people today seem to believe was necessary to see any gains

              ... but is that true? Do you really need all that stuff?

              The Australian researchers probably had a similar question in their minds, when they recruited their 15 strength-trained male field and court sport athletes (mean age 21.7years; 1-RM squat 133.0kg, bench press, 94.7 and 3.1 ± 0.3 years of strength training experience) and randomized them to ingest either a placebo supplement or a supplement "stack" consisting of 15g of Musashi Reactivate Hardcore before, 30g of Musashi Elevator during and 50g of Musashi SPORTS after the workout.
              Table 1: Ingredient profile of the "tri-phasic" peri-workout supplement;  the placebo contained an aspartame based flavor that matched the taste of the active supplement (based on data from Bird. 2013)
              In view of the fact that this probably sounds similarly "Chinese" to you as to me, I've provided you with a tabular overview of the ingredient profile of what the scientists call a "triphasic multinutrient supplement". Basically nothing you would not find in the line-up of every major supplement company: Some carbs, EAAs, creatine, beta alanine and AAKG, caffeine and b-vitamins (not listed in table 1) before workout, carbs, EAAs and creatine intra-workout and the obligatory protein- (whey/casein mix), carb-combination garnished with some creatine and glutamine after the workout. Add in some salt, magnesium and potassium and you can even at the as of late obligatory "contains electrolytes" blend and you are good to go.

              Acute effects = stat. significant, long-term physiological significance = ?

              Caffeine (pre, only), creatine, EAAs, whey (post, only) and even beta alanine and AAKG, the additional carbhydrates, ... all that should do something right? Yep, you are of course right it should. After all people are paying with their hard-earned money for it!
              Figure 1: Serum markers glucose, AST, CRP, CK, cortisol and testosterone before, during, right after, 30min after and 24h after the workouts with the active (SUPP) or placebo beverage (PLA); data expressed relative to group-specific pre-values (Bird. 2013)
              That being said, the data in figure 1 provides at least initial relieve. There are statistically significant effects for almost all measured hormonal parameters,  if we compare the supplemented with the non-supplemented trials in this double-blind, placebo (PLA)-controlled, crossover study.

              Figure 2: Supplement & blood draws (top); overview of the exercises, set x rep scheme and equipment used in the lower body workout (Bird. 2013)
              The study design allowed for a 7-day washout before the "crossing" took place and those participants who had been randomized to the supplement group after an obligatory 28-day washout to clear all previously used supplements (and what not ;-) from the system had to perform the test workout (4 sets of 8 to 15 repetitions for 5 lower-body exercises; see figure 2) with/without the "triphasic nutrient supplement" on top of their 33.6 ± 1.8 kcal/kg body mass diet (macros: 3.8g/kg, 1.5g/kg carbs and protein, respectively.

              The supplement, or rather the supplements, had to be ingested 15 minutes preexercise, in small, regular doses during the exercise, and the whole 300ml of their post-workout drink right after the workout. and thus according to the manufacturers suggestions.

              Aside from the hormonal and inflammatory response (see figure 1) to the workout the scientists also measured the muscular performance and perceptual response during the workouts:
              Figure 3: Perceptual measures of exertion & muscle soreness (Bird. 2013)
              • the total training volume was higher for SUPP (15 836 ± 518 kg⋅repetitions) compared with PLA (14 390 ± 491 kg⋅repetitions) (P <.05;d = 0.70); 
              • countermovement jump peak power (CMJ) did not differ between groups at any timepoint (P> .05;d= 0.05-0.18); in the SUPP group there was however a trend (P= .08;d= 0.32) for increased countermovement jump peak power was yet observed on the third of the four tests 30min after the workout (exact timing see figure 2, top)
              • the global rate of perceived exertion (RPE) did increase after the workout in both groups, and was higher 30min after the supplement trial (P < .01; d= 0.89)
              • the perceptual responses for muscle soreness was elevated and did not differ between treatments
                after the workouts
              However, even for the statistical significant inter-group difference in msucle perceived exertion, the overall effect size is pretty small. It is therefore by no means "obvious", whether the 10% lower perceived exertion during the placebo trial which is probably a direct result of the +10% increase in total training volume, anyways, is of physiological relevance.



              So what do we make of these results? On the one hand it is unquestionably true, that the peri-workout supplementation (I refuse to keep using the hilarious sciency expression "triphasic") did increase the total workout volume, It is also true that it did lower the cortisol increase and produced lower areas under the curve for creatine kinase, but it also lowered the testosterone response to the workout (that the post-workout increase in testosterone is not a legitimate predictor of muscle growth is something you should now, after countless articles on the matter, be aware of; read more about testosterone).

              Whey is more insulinogenic than white bread and creatine could make you fatt True for the 1st, remotely possible for the 2nd! And still both simply work....
              Much ado about nothing? No, not really. While the hormonal changes are more or less irrelevant an increase in total volue that does not entail greater muscle damage could in fact make a difference that's not just statistically, but also physiologically significant.

              What I do yet doubt is that the same results could not have been achieved with a cup of oatmeal, water and protein powder 1h before the workout, a coffee right before the workout and 3-5g of creatine, two bananas and a regular whey protein afterward. All you would have to buy then is a pouch of whey and a 500g jar of creatine monohydrate, which will last you for months. Both SuppVersity supplement staples, as you know and actually among the few supps with physiologically significant effects almost every trainee can benefit from... and did I mention that they are dirt cheap and can be combined with real foods?

              References:
              • Bird SP, Mabon T, Pryde M, Feebrey S, Cannon J. Triphasic multinutrient supplementation during acute resistance exercise improves session volume load and reduces muscle damage in strength-trained athletes. Nutrition Research. April 2013 [EPub ahead of print].

              Leucine Inhibits Nitric Oxide & Beneficial Effects of Cardio Training on Glucose Management. Plus: No Increase in Protein Synthesis W/ Protein After Eccentric Workouts?

              Pre-, Post and Intra-workout supplements are a multi-million dollar business. But are the products at least half as effective as the shiny advertisements claim?
              Let me guess, the actual reason you want to read this article is the shocking claim that everybody's darling, the "pro-anabolic amino acid" leucine may, in addition to having beneficial effects on the phosphorylation of mTOR and the subsequent increase in skeletal muscle protein synthesis, also have negative effects. Right? Ok, I will add a link that takes you right to my elaborations on two recent studies from the São Paulo University and the China Agricultural University and allows you to skip past the information about another recent study.

              A study from the Department of Public Health at the Aarhus University and a study that raises the question, whether its results are indicate that eccentric training is potent enough to maximize the protein anabolic response to an extend that the additional provision of dietary protein will not lead to further increases in markers of protein synthesis.
              You can learn more about protein intake at the SuppVersity

              Protein Timing DOES Matter!

              5x More Than the FDA Allows!

              Protein requ. of athletes

              High EAA protein for fat loss

              Fast vs. slow protein

              Less Fat, More Muscle!
              Before we draw any conclusions, I guess we should first take a look at what Stine Klejs Rahbek and her colleagues actually did: To investigate the effects of leucine-rich whey protein hydrolysate and carbohydrate (WPH+CHO) versus isocaloric carbohydrate (CHO) supplementation on the Akt-mTOR and the AktFOXO signaling axis, during recovery from muscle-damaging exercise and to evaluate whether their hypothesis that WPH+CHO would accentuate signaling for protein synthesis and attenuate signaling for protein degradation, compared to isocaloric CHO, the researchers recruited twenty-four young healthy recreationally active men who had not participated in systematic resistance training or eccentric dominated activities for lower extremity muscles within 6 months prior to inclusion in the study.

              The study itself was conducted in a double blinded, isocaloric placebo-controlled fashion in regards to dietary supplementation. Following inclusion, subjects were randomly allocated into either
              • a whey protein hydrolysate+carbohydrate group (WPH+CHO, n = 12) or
              • isocaloric carbohydrate placebo group (CHO, n = 12)
              On the exercise trial day, subjects reported to the laboratory at 07.30 am in a fasted state. Before the eccentric exercise protocol was initiated, muscle soreness was evaluated using a visual analog scale, a blood sample was collected and knee extensor muscle contractile function was evaluated. Subsequently, a unilateral eccentric exercise protocol for was completed, lasting approximately 30 min (see below).
              Figure 1: Timeline of interventions and measurements on the four study days are shown. A muscle biopsy was sampled 14 days prior to the exercise trial (i.e. to establish basal level). The protocol for days 1 and 2 was identical (Rahbeck. 2015).
              Immediately after the exercise bout, the subjects ingested either a WPH+CHO or a CHO supplement, according to the group they were assigned, and then rested for 3 h. At 3-h post-exercise, a biopsy was obtained from both the exercise and the non-exercise control leg. Before leaving the laboratory, the subjects ingested the second drink (1.00 pm) and received a third drink to ingest 3 h later (4.00 pm).
              "On days 1 and 2 (24 and 48 h following exercise, respectively), the subjects were instructed to ingest three supplements at absolute time points similar to day 0, with the fist drink always ingested after the functional tests and biopsy sampling. Biopsy sampling from both the ECC and the CON leg on days 1 and 2 were performed under conditions similar to the pre-exercise biopsy, i.e., the subjects fasted overnight and rested in the supine position for 45 min prior to biopsy sampling. The biopsy sampling on days 1 and 2 was timed to correspond to 24 and 48 h following exercise termination. Assessments on indices of muscle damage (muscle force, muscle soreness and plasma muscle creatine kinase) were repeated at 24, 48, 72, 96, and 168 h after overnight fasting." (Rahbeck. 2015)
              All Subjects were instructed to refrain from physical activity such as exercise, stair case walking and other types of strenuous activity in the hours/ days between post-exercise assessments of indices of muscle damage to ensure that all measured effects made actually reflected the effects of the exercise + supplementation intervention.
              Figure 2: Effects of eccentric training + supplementation on markers of protein anabolism (left, green) and catabolism (right, red) as measured in the trained leg (Rahbeck. 2015).
              Speaking of effects: If you look at the data in Figure 2, you will realize that the eccentric training led to significant decreases in muscle force (by 23–27 % at 24 h post-exercise), which were followed by gradual, although not full recovery at 168 h post-exercise, with no differences between supplement groups. Furthermore, the phosphorylation of mTOR, p70S6K and rpS6 (=the activity of these protein anabolic proteins) increased and phosphorylation of FOXO1 and FOXO3 (=the activity of these catabolic proteins) decreased in the ECC leg, again with no differences between supplement groups.
              Eccentric training impairs glucose sensitivity in healthy men (Asp. 1995).
              Speaking of eccentric training, you are aware that this form of allegedly highly anabolic (this is scientifically not proven) training will induce a transient decrease in insulin sensitivity (Kirwan. 1992; Asp. 1995), right? So carb binging after an eccentric workout is probably worse than doing the same at any other time for healthy men and women with a decent baseline insulin sensitivity.
              If this is not your first visit to the SuppVersity you will yet know that these observations are not sufficient to warrant the previous voiced hypothesis that the exercise induced increase in protein synthesis in response to eccentric exercises realizes as long as you are training "hard enough", because mTOR, p-p70S6K & co are no reliable measure of the actual amount of protein that's transported into the muscle after the workout.

              Don't freak out about the Rahbeck study, take a look at the latest studies leucine science!

              Instead of freaking out that your protein supplements may be useless, you may thus rather discard this study as interesting, but inconclusive and take a closer look at the latest evidence that leucine, when consumed in excess and isolation could have side effects you may want to avoid.
              • Leucine + endurance exercise - no perfect match? In their latest study, scientist from the São Paulo University were able to show that "leucine supplementation did not potentiate the effects of endurance training on protein turnover, and it also reduced its positive effects on glucose homeostasis" (Costa Junior. 2015) - in rodents.

                In view of the fact that the interactions between endurance exercise, leucine supplementation and glucose metabolism have not previously been studies, the scientists analyzed the effects of endurance exercise training plus leucine supplementation on protein turnover and glucose homeostasis in healthy mice.
                Figure 3: Changes in body composition (left), changes in the expression of the catabolic ubiquitin-proteasome protiens (middle) and effects on glucose disappearance index aka effective glucose uptake after the swimming workout (right | Costa Junior. 2015).
                The results you see in Figure 3 tell you three things: (a) Leucine did not have the previously described minor minor beneficial effects on body composition (increases in lean mass are compensated by increases in fat mass); (b) it did not ameliorate the protein turnover during exercise and (c) it did actively blunt the real world increase in glucose uptake after the endurance workout in spite of the fact that changes in marker proteins like AMPK (not shown)would suggest otherwise.

                Reason to panic? No, the effects are not pronounced enough. In view of the fact that the benefits of isolated leucine supplementation and "spiking" other supplements with extra leucine are totally overblown, anyway. This may be the final straw that brakes the leucine guzzling camel's neck for those of you who's primary goal is to use exercise to improve their glucose tolerance.
              • Leucine an "anti-pump", "anti-heart health" supplement? While they are a bit removed from human in vivo studies, the claims made by Yang et al. in their latest paper in Amino Acids are a bit frightening.

                Based on the observation that increased concentrations of l-leucine in the plasma occur in obese humans and other animals with vascular dysfunction, the scientists argue that the unique inhibitory effect of leucine on NO synthesis from l-arginine in endothelial cells may be part of why the increase in serum BCAA that's brought about by a failure to metabolize the branch-chained amino acids in the obese could negatively modulate cardiovascular homeostasis in insulin resistance.
                Figure 4: This is how leucine messes with NO synthesis. It increases the production of glutamine:fructose- 6-phosphate aminotransferase (GFAT) which then shuts down NO synthesis (Yang. 2015).
                "Results of recent studies indicate that l-leucine is an activator of glutamine:fructose- 6-phosphate aminotransferase (GFAT), which is the fist and a rate-controlling enzyme in the synthesis of glucosamine (an inhibitor of endothelial NO synthesis). Through stimulating the mammalian target of rapamycin signaling pathway and thus protein synthesis, l-leucine may enhance GFAT protein expression, thereby inhibiting NO synthesis in endothelial cells" (Yang. 2015).
                Yang et al. propose that reducing circulating levels of l-leucine or endothelial GFAT activity may provide a potentially novel strategy for preventing and/or treating cardiovascular disease in obese and diabetic subjects and highlight:
                "Such means may include dietary supplementation with either α-ketoglutarate to enhance the catabolism of l-leucine in the small intestine and other tissues or with N-ethyll-glutamine to inhibit GFAT activity in endothelial cells" (Yang. 2015).
                If the scientists (reasonable) assumptions are accurate, anything that prevents the leucine-induced activation of GFAT, be it nutritional supplements or pharmaceutical drugs, may in fact contribute to improved cardiovascular function by enhancing vascular NO synthesis. For the average trainee that's not really relevant, but if you look at the composition of contemporary N.O. boosters this revelation may explain why the "old" NO Xplode with arginine, caffeine & co worked significantly better than its BCAA-laden successors. 
              Study Says "BCCAs, Don't Build Muscle!" I Say "True, But They Seem to Create an Anabolic Potential." | more
              Bottom line: None of the studies presented in this research summary indicates that you have to stop taking the respective supplements. Specifically the use of protein supplements after resistance training workouts is a tried and proven way of augmenting muscle growth - irrespective of the questionably conclusions Stine Klejs Rahbek draw based solely on markers of protein synthesis and in the absence of measuring the influx of protein into the muscle after the standardized eccentric exercise protocol.

              Similarly, the results Yang et al. and Costa Junior et al. present in their papers prove that the incredible hype surrounding leucine is misplaced. They do not, however, provide bullet proof evidence of side effects that are severe enough to flush your leucine and BCAA supplements down the toilette | Comment on Facebook!
                References:
                • Asp, Sven, Jens R. Daugaard, and Erik A. Richter. "Eccentric exercise decreases glucose transporter GLUT4 protein in human skeletal muscle." The Journal of physiology 482.Pt 3 (1995): 705-712.
                • Costa Junior, et al. "Leucine supplementation does not affect protein turnover and impairs the benefiial effects of endurance training on glucose homeostasis in healthy mice." Amino Acids (2015): Ahead of Print.
                • Kirwan, J. P., et al. "Eccentric exercise induces transient insulin resistance in healthy individuals." J Appl Physiol 72.6 (1992): 2197-202.
                • Rahbek, Stine Klejs, et al. "No differential effects of divergent isocaloric supplements on signaling for muscle protein turnover during recovery from muscle-damaging eccentric exercise." Amino Acids (2015): 1-12.
                • Yang, Ying, et al. "l-Leucine and NO-mediated cardiovascular function." Amino acids (2015): 1-13.