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marylin monroe
Showing posts with label calories. Show all posts
Showing posts with label calories. Show all posts

Pistachio Phenols 90% Bioavailable. Phe, Leu, Glu & Trp - Four Satiating Aminos. Artificial Sweeteners Act as Anti- Convulsants. Dendrobium for Glucose, Lipids & Kidneys

Since I am a little pressed in time, I won't beat around the bush, but rather get to the meat of the matter of this Nutrition Quickie, right away... well, actually today's nutrition quickie has no item on meats, but is has one Dendrobium, which is actually rather a supplement - be that as it may, here you go:
  • Since you (hopefully ;-) haven't swallowed a bomb colorimeter, it's actually no wonder that your body can only access 75% and 95% of the energy this little oven can squeeze out of almonds and pistachios. If you went with the Atwood factor (which says fat = 9kcal/g) and simply added fats, proteins and carbs the discrepancy for almonds would even increase to ~35% (Nowotny. 2012)
    Almonds deliver 25% less calories that the nutritional label will tell you (Gebauer. 2012) -- At the FASEB meeting in April 2012, already, scientists from nowhere else than the mighty USDA, respectively the Beltsville Human Nutrition Research Center of the USDA presented the results of a human study that clearly shows that our tummies cannot squeeze out more than 75% of the nutrients a bomb calorimeter does. Nutrition Quickie: 25% Less Kcal in Almonds Than Label Says.

    The caloric value on both food labels and respective nutrient tables is thus off 25% too high. And the corrected energy content per 100g of almonds is 456kcal/100g not 575kcal/100g (nutritiondata.com), or even higher values you will find when you google  "almonds kcal" - I am curious if at least the nutrition labes will ever be updated.

    A similar but less pronounced mismatch has been found for pistachios by the same researchers earlier this year, already. According to a paper published in the January edition of the British Journal of Nutrition (Bear. 2012), the actual energy content of these heart healthy nuts 565kcal/100g, which is ~5% less than the currently established value. 

  • Bioavailability of pistachio polyphenols, xanthophylls, and tocopherols is very high - until you put them into a muffin (Mandalari. 2013) -- Bioavailability, i.e. the ratio of the total amount of a certain molecule that's in the food we eat in intact or at least active form in our bloodstream, can be a real issue for many of the good things the spectral analyzer of brainy scientists detects in our foodstuff.

    Table 1: Phenol, lutein and tocopherol content of the raw, roasted and salted pistacchios and regular and pistaccio (17g/100g) muffins (Madalari. 2012)
    In the upcoming January issue of Nutrition a group of researchers from the UK and Italy report the results of an investigation into the bioavailability of polyphenols, xanthophylls (lutein), and tocopherols (among them the rare gamma-variety) from raw pistachios, roasted salted pistachios, and muffins made with raw pistachios. You can see the original polyphenol, xanthophyll and tocopherol (mind the 90% gamma-tocopherol content, which has better chemoprotective effects than alpha-tocopherol; see "Vitamin(S!) E" post from 2011) content in table 1.

    Interestingly enough the availability of the tocopherols was almost identical for all three tested forms (raw, roasted, in muffins) and even the muffin reduced only the bioaccessability of protocatechuic acid (78%) and luteolin (36%), the rest of the phenols achieved the same ~90% Madalari et al. observed for the raw and roasted + salted pistachios in their million dollar model of the human digestive tract (click here for an article about this "artificial gut")

    • When the gut "tastes" phenylalanine (PHE), leucine (LEU), glutamate (GLUT) and tryptophane (TRP), satiety ensues (Daly. 2012) -- In their most recent paper Christin Daly et al. report on the cholecystokinin (CCK) release in the gut. According to the scientists from the University of Liverpool (UK) and the Kyushu University (Japan), the effect is mediated by interactions with the gastrointestinal bitter taste receptors. Since CCK inhibits food intake and reduces appetite, this provides another mechanistic explanation for the satiety promoting effects of high protein intakes. The effects was observed only for the L- and not the D-amino acids.

      Interestingly, the beneficial effects of  PHE, LEU and GLUT on CCK (but not the TRP-stimulated CCK secretion) were blunted in the presence of gurmarin. "Gurmarin?" Yeah, that's the rodent specific  sweet taste inhibitor in Gymnema sylvestre (note gurmarin does not work in humans; cf. Sigoillot. 2012), which is sold as an anti-diabetes supplement. Inosine, on the other hand increased the CCK release n response to all of the amino acids.

      How significant that is specifically for those who have a problem keeping their ravenous appetite in check is however questionable. After all the satiety response to CCK has been shown to be disturbed (at least in rodents; cf. Balaskó. 2012)

    • Table 2: Number of mice protected by the administered drug in the MES test (Talevi. 2012)
      Acesulfame potassium, cyclamate and saccharin are potential anti-convulsants (Talevi. 2012) -- It may sound counter-intuitive in view of all the bad stuff you have probably heard about artificial sweeteners, but it is their particular molecular structure and similarities between the T1R3 sweet taste receptor they are supposed to bind to ant several metabotropic glutamate receptors from different species that is probably behind the anticonvulsant effects a group of researchers from the Department of Biological Sciences at the Faculty of Exact Sciences of the National University of La Plata (UNLP) in Buenos Aires, Argentina.

      The overall effect size the scientists observed in their rodent model (see table 2) is yet far from earth shattering and generally more pronounced if the sweeteners had been ingested 4h instead of just 20 min before a Maximal Electroshock Seizure (MES) test. Whether this makes them worth "supplementing" is however more than questionable.

      Suggested additional reads:

    • Putting things into perspective: While the DPPH radical scavenging activity is not a really good measure of the in-vivo anti-oxidant potency of a given molecule it may yet still be worth mentioning that the one of Dendrobium (IC50 = 29.6 μg/mL) is more than 80% lower than that Areca catechuvar. Cinnamon cassia, Paeonia suffruticosa and Alpinia officinarum extracts which share IC50 values <6µg/mL (Lee. 2003). In other words, you need 80% more Dendrobium than cinnamon, for example, to neutralize the same amount H2O2 radicals.
      Dendrobium extract ameliorates renal fat accumulation, hyperglycemia and hyperlipidemia in rodents on "high fat" diet (Lee. 20012) -- While I cannot say if this is the same Dendrobium extract that's used in a pre-workout supplement that's "all the craze", these days, I can tell you that a group of researchers just published a paper on the renoprotective, hypoglycemic and hypolipidemic effects of an extract from Dendrobium moniliforme (a cursory search revealed that even within this genus of orchids there are at least 90 sub-types ranging from Aochidori to Yuten).

      Lee et al. administered the methanolic extract at dosages of 200mg/kg (HED ~16mg/kg) for nine weeks and reduced the elevated serum glucose, total cholesterol concentration and renal lipid accumulation in the HFD-fed mice. It also ameliorated renal dysfunction biomarkers including serum creatinine and renal collagen IV deposition. So that the scientists conclude that methanolic extracts from Dendrobium moniliforme exhibit pleiotropic effects on obesity induced parameters and exert renoprotective effect in HFD-fed mice.


    That's it for today,
    unless you are are interested in one of the following facebook news:
    • Crape ginger (Costus speciosus Koen) has significant anti-arthritic properties - at least in a rodent model (read more)
    • Ayurvedic polyherbal Unani formulation shows promising results in Acne vulgaris patients - 45 days, 45 subjects, significant improvements on Cook's acne scale (read more)
    • Endocannabinoids increase, leptin decreases a "sweet tooth" - And you can take this almost literally, since they do actually modulate sweet taste receptor sensitivity (read more)
        There are actually a couple more and they will "proliferate" *rofl* even before the next official SuppVersity  post will be published. Reason enough to check by from time to time, or simply "like" the SuppVersity on Facebook in order to keep up with the news.


        References:
        • Baer DJ, Gebauer SK, Novotny JA. Measured energy value of pistachios in the human diet. Br J Nutr. 2012 Jan;107(1):120-5.
        • Balaskó M, Soós S, Párniczky A, Koncsecskó-Gáspár M, Székely M, Pétervári E. Anorexic effect of peripheral cholecystokinin (CCK) varies with age and body composition (short communication). Acta Physiol Hung. 2012 Jun;99(2):166-72.
        • Gebauer SK, Novotny JA, Baer DJ. Macronutrient absorption from almonds: the measured energy value of almonds in the human diet. FASEB Journal. 2012;26:820.25.
        • Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life Sci. 2003 May 30;73(2):167-79.
        • Lee W, Eom DW, Jung Y, Yamabe N, Lee S, Jeon Y, Hwang YR, Lee JH, Kim YK, Kang KS, Kim SN. Dendrobium moniliforme Attenuates High-Fat Diet-Induced Renal Damage in Mice through the Regulation of Lipid-Induced Oxidative Stress. Am J Chin Med. 2012;40(6):1217-28.
        • Mandalari G, Bisignano C, Filocamo A, Chessa S, Sarò M, Torre G, Faulks RM, Dugo P. Bioaccessibility of pistachio polyphenols, xanthophylls, and tocopherols during simulated human digestion. Nutrition. 2013 Jan;29(1):338-44.
        • Novotny JA, Gebauer SK, Baer DJ. Discrepancy between the Atwater factor predicted and empirically measured energy values of almonds in human diets. Am J Clin Nutr. 2012 Aug;96(2):296-301. 
        • Sigoillot M, Brockhoff A, Meyerhof W, Briand L. Sweet-taste-suppressing compounds: current knowledge and perspectives of application. Appl Microbiol Biotechnol. 2012 Nov;96(3):619-30.
        • Talevi A, Enrique AV, Bruno-Blanch LE. Anticonvulsant activity of artificial sweeteners: a structural link between sweet-taste receptor T1R3 and brain glutamate receptors. Bioorg Med Chem Lett. 2012 Jun 15;22(12):4072-4.

        High Reps vs. 5x5 - Revisiting the "High(er) Reps for Fat Loss"-Myth: Do You Really Believe that "Burning" +13 Extra Calories Will Make a Difference?

        Image 1: Vince Andrich, here at the 1988 Nevada State Bodybuilding Competition, knew it all along: Hard work, not high reps will get you the stage-ready physique everybody aspires... and I mean look at him are you seriously questioning Vince's expertise?
        If you have listened to the latest installments of BodyRX Radio, the idea that strength (and HIIT) training, not calorie restriction and endless cardio sessions pave the way to a leaner, more muscular physique. And although it may be of secondary importance whether you are burning 100kcal or 200kcal during those workouts, it stands out of question that you won't get rid of those damn spare tire, if you do not exert yourself in the gym (something Layne Norton is notorious for, as you may have seen in one of his workout videos or heard on BodyRX, lately) - because, after all, energy expenditure does count, even if the energy equation is much more complex than the simplistic calories in vs. calories out paradigm that is still upheld by mainstream dietitians. The results of a recent study from scientists from the Departments of Health and Sport Sciences at the Salisbury University and the University of South Carolina are may thus come handy to decide, to which extent workout intensity and volume influence acute and post-exercise energy expenditure (Mazetti. 2011). Or, to make it simple, does the good old bro-scientific high volume, high rep, low-weight training during a contest prep make any sense at all?

        Exposed! The absurdity of going to the gym to "burn calories"

        Scott A. Mazetti and his colleagues recruited 10 resistance trained young men (22+/-3.6 years) with an average body mass of 84+/-6.4kg, a height of 180+/-5.1cm, and a body fat percentage of 13+/-3.8% - an adequate model of the "average gymrat", if you asked me. After a 3-week familiarization and testing period, all participants performed every of the following four different explosive strength training regimen in a randomly assigned, but counterbalanced order (cf. figure 1)
        Figure 1: The four training protocols all trainees performed after an initial 3-week familiarization and testing period in a randomly assigned, but counterbalanced order (Mazetti. 2011)
        Now, you may complain that there is neither a "high rep" nor a "heavy group" in the conventional sense of 15+ pump training with an endless amount of sets, or the minimalist 1-2 rep approach of the hardcore HIT faction... granted, you are right. Nevertheless, we should see a significant difference in energy expenditure between a 5x5 and a 4x10 (both normally considered as "hypertrophy training) regimen, already, if the good old saying "high(er) reps" for increased energy expenditure and subsequent fat loss had any merit.
        Figure 2: Energy expenditure (kcal/min) during and after squatting, and bench pressing in the four training groups and total energy expenditure during the whole workout and 60min post-workout window (data adapted from Mazetti. 2011)
        As figure 2 goes to show, there are differences in energy expenditure between the different protocols, during the squat, the deadlift and up to 5 minutes post exercise, nevertheless, the "the differences in total energy expenditure among protocols were not significant". So, even if there were any merit in exercising primarily to burn calories during your workout (a ridiculous way of trying to lose weight, which is predestined to fail, anyway), the -6kcal difference between the -13kcal difference between the least energy consuming form of training, i.e. 5x5 and the one with the highest energy demand, i.e. 4x10, would not even suffice to "make up" (another hilarious idea) for proverbial "apple à day", which keeps the doctor away.
        Image 2: Don't let your lazy love handles decide what type of "cardio" you are doing!
        A brief note on what Lane already pointed out in the last installment of Body RX Radio: Classic low-intensity cardio training may "burn" calories for a week or two. Afterwards, this type of chronic low-grade stressor is yet notorious for shutting down your metabolism and reducing your resting energy expenditure. Of high intensity aerobic exercise with intensities way beyond the 70% VO2max limit (YES! This is aerobic, two - cf. "HIIT is the Hit! Even for Patients with Myocardial Infarctions!"), we have known for decades that it increases your resting energy expenditure by "5 ±15% for 24 ± 48 h" (Hunter. 1998). So, I suggest you get off the stationary bike you are just riding while browsing the web, get your running shoes out and do a couple of sprints. Your spare tire won't like that, but I bet you will like the effect it's going to have on the person you see in the mirror, each morning ;-)
        In view of these results, it appears prudent to reassess the often-heard advice of doing high reps for fat loss. Even if you insist that you need to "burn calories" in the gym, it is very unlikely that those few extra calories would make a noticeable difference in terms of how you look on stage - or just in front of your private mirror. Moreover, even this small advantage vanishes as soon, as you increase the workload by doing 2x7 + 2x6 with a heavy weight, or put simply: Identical workload identical calorie expenditure.

        Carbohydrate Restriction Reduces Liver Triglycerides More Effectively Than Calorie Restriction - With Equal Weight Loss Outcomes.

        A calorie is not a calorie, I hope you knew that even before Garry Taubes published his famous NY Times best-seller. Yet, in case you are still looking for respective studies to show to medially indoctrinated family members or friends, who still believe that - even if fat is not the enemy - half ratios would be the key to health & longevity, you might be interested in the results of a very recent investigation by Browning et al. (Browning. 2011).

        The scientists put a group 18 non-alcoholic-fatty-liver patients on either a carbohydrate-restricted (<20g/day) or a calorie restricted (1200-1500kcal/day) diet - just to make sure you get this right: the carb-restricted group ate as much calories as they wanted! - and measured hepatic triglyceride levels before and after the 2 week dietary intervention. Probably to the surprise of many members of the medical orthodoxy, both groups lost about the same amount of weight (in fact the low-carb group lost -4.6kg, while the low-calorie group lost only -4.0kg!), yet the low-carb group had a much greater reduction in hepatic triglyceride levels:
        Liver triglycerides decreased significantly with weight loss (P , 0.001) but decreased significantly more (P = 0.008) in carbohydrate-restricted subjects (-55.6%) than in calorie-restricted subjects (-28.6%).
        So, even if you are not particularly interested in your liver health, the fact that by eating to satiety and just dropping your carb intake to <20g/day you can lose the same ~4.5kg you would lose if you starved yourself for 14 days should prick your ears.

        Burn Additional 27 kcal with 6mg/kg Caffeine Pre-Workout

        Caffeine is the cornerstone of almost each and every fat-burner on the market. But how effective is it in raising your energy expenditure? A study (Astorino. 2011) conducted at the Department of Kinesiology of the California State University provides an answer: Not very effective.

        Astorino et al. had 14 strength-trained men who were regular caffeine consumers perform a strength workout with either 6mg/kg (thanks to Ezhan who pointed me to a type here. It's 6mg not 0.6 as it said before) caffeine or placebo supplement pre-workout. What they found was that
        Caffeine intake increased total energy expenditure by 15% (P<0.05), but the additional calories burned was minimal (+27 kcal).
        Well, I suppose this is about the amount of calories of a small carrot and not really what you would expect from a fat burner.